ASBL machine-compatible
Biofloppy contains human-derived organoids of one cell type with culture and maintenance protocols built in. Biofloppy is inserted into ASBL machines to use.
An organoid (1,2) is a small clump of living cells. It survives independently if provided with nutrients and waste clearance. US FDA is replacing animal trials for drugs with organoids and organ-on-a-chip systems.
ASBL machines use biofloppies. ASBL machines have in-built functions (in ASBL Machine Protocol) to provide nutrients to and extract waste from organoid microenvironments. An ASBL organoid chip is called a biofloppy, and used like a floppy disk.
Biofloppy Usage
1. Insert a biofloppy with organoids in the biofloppy drive of an ASBL machine
2. ASBL recognizes the biofloppy and displays media requirements
3. Insert a 96-well plate with required media into the ASBL machine for organoid maintenance
4. Access biofloppy applications with ASBL terminal
Biofloppy Applications
1. Create patient organoids from autologous (derived from patient) cells
2. Test personalized therapies on patient organoids in cancer and autoimmune diseases
3. Develop organ-targeting and cell-targeting therapies with parallel assays on multiple biofloppies
4. Cell therapy research and manufacturing
5. Transfer wetware
Available Biofloppies (order by email)
1. Bone marrow biofloppy
2. Cardiac muscle biofloppy
3. Liver hepatocyte biofloppy
4. Lung biofloppy
5. Neuron biofloppy
6. Intestinal biofloppy
7. Endometrium biofloppy
8. Skin epithelium biofloppy
9. Empty biofloppy (create and save organoids)
Deconstructed Human is a biofloppy package by ASBL with one of all available biofloppies, 96-well plates with 90-day media for all biofloppies, and organoid manipulation training on ASBL machines.
Genetic Sequencing Unit is a sequencing add-on for ASBL machines like GPU (Graphics Processing Unit) for PC (personal computer)
Microfluidic unit sequences DNA post PCR. Flow cell size. GSU is being designed to be reprogrammable for all applications listed below to probe diverse biology.
Application (reference): biology probed
DNAseq (whole genome sequencing): genome
WES (whole exome sequencing): protein-coding genes (exons)
RNAseq (bulk RNA sequencing): active mRNA across bulk tissue (many cells), transcriptome
scRNAseq (single-cell RNA sequencing): active mRNA in single cells, active genes, single-cell transcriptome
ATACseq: chromatin accessibility, epigenome
Hi-C: chromatin position, long-range contact like genes and regulatory elements, transcription regulation
EM-Seq (Enzymatic methyl-seq): DNA methylation
ChIPseq (chromatin immunoprecipitation massively parallel sequencing): binding sites of proteins on DNA
16S rRNAseq (16S ribosomal RNA sequencing): bacterial identification
ITS-seq (Internal Transcribed Spacer sequencing): fungal and bacterial identification
Bioengineers can add sequencing applications like CITE-seq and SMART-seq by reprogramming GSU using ASBL Machine Protocol
Matrixims is a digital world where avatars (non-player characters or NPCs) are controlled by brain cells in a neural organoid biofloppy chip. Neuronal organoids (1) play The Sims.
Read more about Matrixims here